Desai, A. & Mitchison, T. J. Microtubule polymerization dynamics. Annu. Rev. Cell Dev. Biol. 13, 83–117 (1997).

CAS  PubMed  Google Scholar 

Mastronarde, D. N., McDonald, K. L., Ding, R. & McIntosh, J. R. Interpolar spindle microtubules in PTK cells. J. Cell Biol. 123, 1475–1489 (1993).

CAS  PubMed  Google Scholar 

Schroeder, T. E. Cytokinesis: filaments in the cleavage furrow. Exp. Cell Res. 53, 272–276 (1968).

CAS  PubMed  Google Scholar 

Schroeder, T. E. Actin in dividing cells: contractile ring filaments bind heavy meromyosin. Proc. Natl Acad. Sci. USA 70, 1688–1192 (1973).

CAS  PubMed  PubMed Central  Google Scholar 

Mabuchi, I. & Okuno, M. The effect of myosin antibody on the division of starfish blastomeres. J. Cell Biol. 74, 251–263 (1977).

CAS  PubMed  PubMed Central  Google Scholar 

Knecht, D. A. & Loomis, W. F. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science 236, 1081–1086 (1987).

CAS  PubMed  Google Scholar 

De Lozanne, A. & Spudich, J. A. Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science 236, 1086–1091 (1987).

CAS  PubMed  Google Scholar 

Straight, A. F. et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299, 1743–1747 (2003).

CAS  PubMed  Google Scholar 

Wheatley, S. P. & Wang, Y. Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells. J. Cell Biol. 135, 981–989 (1996).

CAS  PubMed  Google Scholar 

Powers, J., Bossinger, O., Rose, D., Strome, S. & Saxton, W. A nematode kinesin required for cleavage furrow advancement. Curr. Biol. 8, 1133–1136 (1998).

CAS  PubMed  PubMed Central  Google Scholar 

Raich, W. B., Moran, A. N., Rothman, J. H. & Hardin, J. Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4. Mol. Biol. Cell 9, 2037–2049 (1998).

CAS  PubMed  PubMed Central  Google Scholar 

Jantsch-Plunger, V. et al. CYK-4: A Rho family GTPase activating protein (GAP) required for central spindle formation and cytokinesis. J. Cell Biol. 149, 1391–1404 (2000).

CAS  PubMed  PubMed Central  Google Scholar 

Eggert, U. S., Mitchison, T. J. & Field, C. M. Animal cytokinesis: from parts list to mechanisms. Annu. Rev. Biochem. 75, 543–566 (2006).

CAS  PubMed  Google Scholar 

Barr, F. A. & Gruneberg, U. Cytokinesis: placing and making the final cut. Cell 131, 847–860 (2007).

CAS  PubMed  Google Scholar 

Werner, M. & Glotzer, M. Control of cortical contractility during cytokinesis. Biochem. Soc. Trans. 36, 371–377 (2008).

CAS  PubMed  Google Scholar 

Somers, W. G. & Saint, R. A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev. Cell 4, 29–39 (2003).

CAS  PubMed  Google Scholar 

Yuce, O., Piekny, A. & Glotzer, M. An ECT2-centralspindlin complex regulates the localization and function of RhoA. J. Cell Biol. 170, 571–582 (2005).

PubMed  PubMed Central  Google Scholar 

Nishimura, Y. & Yonemura, S. Centralspindlin regulates ECT2 and RhoA accumulation at the equatorial cortex during cytokinesis. J. Cell Sci. 119, 104–114 (2006).

CAS  PubMed  Google Scholar 

Kamijo, K. et al. Dissecting the role of Rho-mediated signaling in contractile ring formation. Mol. Biol. Cell 17, 43–55 (2006).

CAS  PubMed  PubMed Central  Google Scholar 

Dechant, R. & Glotzer, M. Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation. Dev. Cell 4, 333–344 (2003).

CAS  PubMed  Google Scholar 

Bringmann, H. & Hyman, A. A. A cytokinesis furrow is positioned by two consecutive signals. Nature 436, 731–734 (2005).

CAS  PubMed  Google Scholar 

Werner, M., Munro, E. & Glotzer, M. Astral signals spatially bias cortical myosin recruitment to break symmetry and promote cytokinesis. Curr. Biol. 17, 1286–1297 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Murthy, K. & Wadsworth, P. Dual role for microtubules in regulating cortical contractility during cytokinesis. J. Cell Sci. 121, 2350–2359 (2008).

CAS  PubMed  Google Scholar 

Piekny, A., Werner, M. & Glotzer, M. Cytokinesis: welcome to the Rho zone. Trends Cell Biol. 15, 651–658 (2005).

CAS  PubMed  Google Scholar 

Heald, R. et al. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420–445 (1996).

CAS  PubMed  Google Scholar 

Kapoor, T. M., Mayer, T. U., Coughlin, M. L. & Mitchison, T. J. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J. Cell Biol. 150, 975–988 (2000).

CAS  PubMed  PubMed Central  Google Scholar 

Tournebize, R. et al. Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nature Cell Biol. 2, 13–19 (2000).

CAS  PubMed  Google Scholar 

Rusan, N. M., Fagerstrom, C. J., Yvon, A. M. & Wadsworth, P. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein–α tubulin. Mol. Biol. Cell 12, 971–980 (2001).

CAS  PubMed  PubMed Central  Google Scholar 

Paweletz, N. Walther Flemming: pioneer of mitosis research. Nature Rev. Mol. Cell Biol. 2, 72–75 (2001).

CAS  Google Scholar 

Saxton, W. M. & McIntosh, J. R. Interzone microtubule behavior in late anaphase and telophase spindles. J. Cell Biol. 105, 875–886 (1987). Provides an early demonstration that central spindle microtubule bundles are unusually stable and that the sliding of bundles accompanies spindle elongation.

CAS  PubMed  Google Scholar 

Skop, A. R., Liu, H., Yates, J., Meyer, B. J. & Heald, R. Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. Science 305, 61–66 (2004).

CAS  PubMed  PubMed Central  Google Scholar 

Gromley, A. et al. Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission. Cell 123, 75–87 (2005).

CAS  PubMed  Google Scholar 

Greenbaum, M. P. et al. TEX14 is essential for intercellular bridges and fertility in male mice. Proc. Natl Acad. Sci. USA 103, 4982–4987 (2006).

CAS  PubMed  PubMed Central  Google Scholar 

Zhao, W. M., Seki, A. & Fang, G. Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesis. Mol. Biol. Cell 17, 3881–3896 (2006).

CAS  PubMed  PubMed Central  Google Scholar 

Carlton, J. G. & Martin-Serrano, J. Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery. Science 316, 1908–1912 (2007).

CAS  PubMed  Google Scholar 

Morita, E. et al. Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 26, 4215–4227 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Goss, J. W. & Toomre, D. K. Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis. J. Cell Biol. 181, 1047–1054 (2008).

CAS  PubMed  PubMed Central  Google Scholar 

Pohl, C. & Jentsch, S. Final stages of cytokinesis and midbody ring formation are controlled by BRUCE. Cell 132, 832–845 (2008).

CAS  PubMed  Google Scholar 

Durcan, T. M. et al. Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis. J. Cell Biol. 181, 595–603 (2008).

CAS  PubMed  PubMed Central  Google Scholar 

Saxton, W. M. et al. Tubulin dynamics in cultured mammalian cells. J. Cell Biol. 99, 2175–2186 (1984).

CAS  PubMed  Google Scholar 

Salmon, E. D., Leslie, R. J., Saxton, W. M., Karow, M. L. & McIntosh, J. R. Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching. J. Cell Biol. 99, 2165–2174 (1984).

CAS  PubMed  Google Scholar 

Shelden, E. & Wadsworth, P. Interzonal microtubules are dynamic during spindle elongation. J. Cell Sci. 97, 273–281 (1990).

CAS  PubMed  Google Scholar 

Rosa, J., Canovas, P., Islam, A., Altieri, D. C. & Doxsey, S. J. Survivin modulates microtubule dynamics and nucleation throughout the cell cycle. Mol. Biol. Cell 17, 1483–1493 (2006).

CAS  PubMed  PubMed Central  Google Scholar 

Bucciarelli, E., Giansanti, M. G., Bonaccorsi, S. & Gatti, M. Spindle assembly and cytokinesis in the absence of chromosomes during Drosophila male meiosis. J. Cell Biol. 160, 993–999 (2003).

CAS  PubMed  PubMed Central  Google Scholar 

Savoian, M. S., Earnshaw, W. C., Khodjakov, A. & Rieder, C. L. Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E. Mol. Biol. Cell 10, 297–311 (1999).

CAS  PubMed  PubMed Central  Google Scholar 

Canman, J. C., Hoffman, D. B. & Salmon, E. D. The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle. Curr. Biol. 10, 611–664 (2000).

CAS  PubMed  Google Scholar 

Alsop, G. B. & Zhang, D. Microtubules are the only structural constituent of the spindle apparatus required for induction of cell cleavage. J. Cell Biol. 162, 383–390 (2003).

CAS  PubMed  PubMed Central  Google Scholar 

Jiang, W. et al. PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis. Mol. Cell 2, 877–885 (1998). The identification of a mammalian orthologue of Ase1, PRC1, and the initial indications for its involvement in cytokinesis.

CAS  PubMed  Google Scholar 

Schuyler, S. C., Liu, J. Y. & Pellman, D. The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins. J. Cell Biol. 160, 517–528 (2003).

CAS  PubMed  PubMed Central  Google Scholar 

Muller, S. et al. The plant microtubule-associated protein AtMAP65–63/PLE is essential for cytokinetic phragmoplast function. Curr. Biol. 14, 412–417 (2004).

CAS  PubMed  PubMed Central  Google Scholar 

Loiodice, I. et al. Ase1p organizes antiparallel microtubule arrays during interphase and mitosis in fission yeast. Mol. Biol. Cell 16, 1756–1768 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Yamashita, A., Sato, M., Fujita, A., Yamamoto, M. & Toda, T. The roles of fission yeast Ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling. Mol. Biol. Cell 16, 1378–1395 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Zhu, C. & Jiang, W. Cell cycle-dependent translocation of PRC1 on the spindle by KIF4 is essential for midzone formation and cytokinesis. Proc. Natl Acad. Sci. USA 102, 343–348 (2005).

CAS  PubMed  Google Scholar 

Mollinari, C. et al. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 157, 1175–1186 (2002). Provides a thorough domain analysis of PRC1, and depletion analysis that demonstrates the role of PRC1 in central spindle assembly.

CAS  PubMed  PubMed Central  Google Scholar 

Kurasawa, Y., Earnshaw, W. C., Mochizuki, Y., Dohmae, N. & Todokoro, K. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 23, 3237–3248 (2004). Demonstration of biochemical and functional links between the kinesin KIF4A and PRC1.

CAS  PubMed  PubMed Central  Google Scholar 

Janson, M. E. et al. Crosslinkers and motors organize dynamic microtubules to form stable bipolar arrays in fission yeast. Cell 128, 357–368 (2007). Demonstration of how the combined activities of PRC1 and a plus-end-directed kinesin slide microtubules to generate bundles.

CAS  PubMed  Google Scholar 

Mishima, M., Kaitna, S. & Glotzer, M. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell 2, 41–54 (2002). Demonstration that CYK4 and MKLP1 form an evolutionarily conserved complex that is required for central spindle assembly.

CAS  PubMed  Google Scholar 

Pavicic-Kaltenbrunner, V., Mishima, M. & Glotzer, M. Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex. Mol. Biol. Cell 18, 4992–5003 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Sellitto, C. & Kuriyama, R. Distribution of a matrix component of the midbody during the cell cycle in Chinese hamster ovary cells. J. Cell Biol. 106, 431–449 (1988). Immunolocalization of a midbody component, subsequently identified as MKLP1, that colocalizes with the electron-dense matrix.

CAS  PubMed  Google Scholar 

Adams, R. R., Tavares, A. A., Salzberg, A., Bellen, H. J. & Glover, D. M. pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis. Genes Dev. 12, 1483–1494 (1998). Pioneering genetic analysis of the role of MKLP1 orthologues in cytokinesis.

CAS  PubMed  PubMed Central  Google Scholar 

Glotzer, M. The molecular requirements for cytokinesis. Science 307, 1735–1739 (2005).

CAS  PubMed  Google Scholar 

Vale, R. D. & Fletterick, R. J. The design plan of kinesin motors. Annu. Rev. Cell Dev. Biol. 13, 745–777 (1997).

CAS  PubMed  Google Scholar 

Rice, S. et al. A structural change in the kinesin motor protein that drives motility. Nature 402, 778–784 (1999).

CAS  PubMed  Google Scholar 

Case, R. B., Rice, S., Hart, C. L., Ly, B. & Vale, R. D. Role of the kinesin neck linker and catalytic core in microtubule-based motility. Curr. Biol. 10, 157–160 (2000).

CAS  PubMed  Google Scholar 

Jeyaprakash, A. A. et al. Structure of a Survivin–Borealin–INCENP core complex reveals how chromosomal passengers travel together. Cell 131, 271–285 (2007). Structural characterization of the CPC revealing that the three proteins co-assemble into a three-stranded helix.

CAS  PubMed  Google Scholar 

Sessa, F. et al. Mechanism of Aurora B activation by INCENP and inhibition by hesperadin. Mol. Cell 18, 379–391 (2005).

CAS  PubMed  Google Scholar 

Earnshaw, W. C. & Cooke, C. A. Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation. J. Cell Sci. 98, 443–461 (1991).

PubMed  Google Scholar 

Vader, G., Kauw, J. J., Medema, R. H. & Lens, S. M. Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody. EMBO Rep. 7, 85–92 (2006).

CAS  PubMed  Google Scholar 

Ban, R., Irino, Y., Fukami, K. & Tanaka, H. Human mitotic spindle-associated protein PRC1 inhibits MgcRacGAP activity toward Cdc42 during the metaphase. J. Biol. Chem. 279, 16394–16402 (2004).

CAS  PubMed  Google Scholar 

Guse, A., Mishima, M. & Glotzer, M. Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Curr. Biol. 15, 778–786 (2005).

CAS  PubMed  Google Scholar 

Neef, R., Klein, U. R., Kopajtich, R. & Barr, F. A. Cooperation between mitotic kinesins controls the late stages of cytokinesis. Curr. Biol. 16, 301–307 (2006).

CAS  PubMed  Google Scholar 

Mackay, A. M., Eckley, D. M., Chue, C. & Earnshaw, W. C. Molecular analysis of the INCENPs (inner centromere proteins): separate domains are required for association with microtubules during interphase and with the central spindle during anaphase. J. Cell Biol. 123, 373–385 (1993).

CAS  PubMed  Google Scholar 

Wheatley, S. P., Carvalho, A., Vagnarelli, P. & Earnshaw, W. C. INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. Curr. Biol. 11, 886–890 (2001).

CAS  PubMed  Google Scholar 

Hill, E., Clarke, M. & Barr, F. A. The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis. EMBO J. 19, 5711–5719 (2000).

CAS  PubMed  PubMed Central  Google Scholar 

Neef, R. et al. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J. Cell Biol. 162, 863–875 (2003).

CAS  PubMed  PubMed Central  Google Scholar 

Abaza, A. et al. M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis. J. Biol. Chem. 278, 27844–27852 (2003).

CAS  PubMed  Google Scholar 

Gruneberg, U., Neef, R., Honda, R., Nigg, E. A. & Barr, F. A. Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2. J. Cell Biol. 166, 167–172 (2004). Demonstration that MKLP2 has a crucial role in mediating the localization of the CPC.

CAS  PubMed  PubMed Central  Google Scholar 

Neef, R. et al. Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1. Nature Cell Biol. 9, 436–444 (2007). Provides a detailed analysis of PRC1 isoforms and demonstrates that the mitotic phosphorylation of PRC1 inhibits the recruitment of PLK1.

CAS  PubMed  Google Scholar 

Jang, J. K., Rahman, T. & McKim, K. S. The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. Mol. Biol. Cell 16, 4684–4694 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Maiato, H. et al. MAST/Orbit has a role in microtubule–kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. J. Cell Biol. 157, 749–760 (2002).

CAS  PubMed  PubMed Central  Google Scholar 

Inoue, Y. H. et al. Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J. Cell Biol. 166, 49–60 (2004).

CAS  PubMed  PubMed Central  Google Scholar 

Gonzalez, C. et al. Mutations at the asp locus of Drosophila lead to multiple free centrosomes in syncytial embryos, but restrict centrosome duplication in larval neuroblasts. J. Cell Sci. 96, 605–616 (1990).

PubMed  Google Scholar 

Wakefield, J. G., Bonaccorsi, S. & Gatti, M. The Drosophila protein Asp is involved in microtubule organization during spindle formation and cytokinesis. J. Cell Biol. 153, 637–648 (2001). One of the few papers that provides functional insight into factors that contribute to central spindle assembly by binding to the minus ends of the bundles.

CAS  PubMed  PubMed Central  Google Scholar 

Bond, J. et al. ASPM is a major determinant of cerebral cortical size. Nature Genet. 32, 316–320 (2002).

CAS  PubMed  Google Scholar 

do Carmo Avides, M., Tavares, A. & Glover, D. M. Polo kinase and Asp are needed to promote the mitotic organizing activity of centrosomes. Nature Cell Biol. 3, 421–424 (2001).

CAS  PubMed  Google Scholar 

Fabbro, M. et al. Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis. Dev. Cell 9, 477–488 (2005).

CAS  PubMed  Google Scholar 

Verni, F. et al. Feo, the Drosophila homolog of PRC1, is required for central-spindle formation and cytokinesis. Curr. Biol. 14, 1569–1575 (2004).

CAS  PubMed  Google Scholar 

Mollinari, C. et al. Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not. Mol. Biol. Cell 16, 1043–1055 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Verbrugghe, K. J. & White, J. G. SPD-1 is required for the formation of the spindle midzone but is not essential for the completion of cytokinesis in C. elegans embryos. Curr. Biol. 14, 1755–1760 (2004).

CAS  PubMed  Google Scholar 

Kieserman, E. K., Glotzer, M. & Wallingford, J. B. Developmental regulation of central spindle assembly and cytokinesis during vertebrate embryogenesis. Curr. Biol. 18, 116–123 (2008).

CAS  PubMed  Google Scholar 

Severson, A. F., Hamill, D. R., Carter, J. C., Schumacher, J. & Bowerman, B. The Aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis. Curr. Biol. 10, 1162–1171 (2000). An influential paper that used temperature-sensitive alleles to demonstrate a functional interaction between the MKLP1 orthologue and Aurora B and to estimate when they act during cytokinesis.

CAS  PubMed  Google Scholar 

Kaitna, S., Mendoza, M., Jantsch-Plunger, V. & Glotzer, M. Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis. Curr. Biol. 10, 1172–1181 (2000).

CAS  PubMed  Google Scholar 

Simon, G. C. et al. Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO J. 27, 1791–1803 (2008).

CAS  PubMed  PubMed Central  Google Scholar 

Santamaria, A. et al. Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis. Mol. Biol. Cell 18, 4024–4036 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Petronczki, M., Glotzer, M., Kraut, N. & Peters, J. M. Polo-like kinase 1 triggers the initiation of cytokinesis in human cells by promoting recruitment of the RhoGEF Ect2 to the central spindle. Dev. Cell 12, 713–725 (2007).

CAS  PubMed  Google Scholar 

Burkard, M. E. et al. Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells. Proc. Natl Acad. Sci. USA 104, 4383–4388 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Johnson, E. F., Stewart, K. D., Woods, K. W., Giranda, V. L. & Luo, Y. Pharmacological and functional comparison of the polo-like kinase family: insight into inhibitor and substrate specificity. Biochemistry 46, 9551–9563 (2007).

CAS  PubMed  Google Scholar 

Blangy, A. et al. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 83, 1159–1169 (1995).

CAS  PubMed  Google Scholar 

Sawin, K. E. & Mitchison, T. J. Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proc. Natl Acad. Sci. USA 92, 4289–4293 (1995).

CAS  PubMed  PubMed Central  Google Scholar 

Saunders, A. M., Powers, J., Strome, S. & Saxton, W. M. Kinesin-5 acts as a brake in anaphase spindle elongation. Curr. Biol. 17, R453–R454 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Mishima, M., Pavicic, V., Gruneberg, U., Nigg, E. A. & Glotzer, M. Cell cycle regulation of central spindle assembly. Nature 430, 908–913 (2004).

CAS  PubMed  Google Scholar 

Zhu, C., Lau, E., Schwarzenbacher, R., Bossy-Wetzel, E. & Jiang, W. Spatiotemporal control of spindle midzone formation by PRC1 in human cells. Proc. Natl Acad. Sci. USA 103, 6196–6201 (2006). References 101 and 102 show that mitotic phosphorylation of MKLP1 and PRC1, respectively, inhibit central spindle assembly during mitosis.

CAS  PubMed  PubMed Central  Google Scholar 

Murata-Hori, M., Tatsuka, M. & Wang, Y. L. Probing the dynamics and functions of Aurora B kinase in living cells during mitosis and cytokinesis. Mol. Biol. Cell 13, 1099–1108 (2002).

CAS  PubMed  PubMed Central  Google Scholar 

Karsenti, E., Nedelec, F. & Surrey, T. Modelling microtubule patterns. Nature Cell Biol. 8, 1204–1211 (2006).

CAS  PubMed  Google Scholar 

Nedelec, F. Computer simulations reveal motor properties generating stable antiparallel microtubule interactions. J. Cell Biol. 158, 1005–1015 (2002). A computational exploration of mechanisms that could generate stable microtubule overlap.

CAS  PubMed  PubMed Central  Google Scholar 

Goshima, G., Nedelec, F. & Vale, R. D. Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins. J. Cell Biol. 171, 229–240 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Sproul, L. R., Anderson, D. J., Mackey, A. T., Saunders, W. S. & Gilbert, S. P. Cik1 targets the minus-end kinesin depolymerase Kar3 to microtubule plus ends. Curr. Biol. 15, 1420–1427 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Burbank, K. S., Mitchison, T. J. & Fisher, D. S. Slide-and-cluster models for spindle assembly. Curr. Biol. 17, 1373–1383 (2007).

CAS  PubMed  Google Scholar 

Mitchison, T. J. et al. Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles. Mol. Biol. Cell 16, 3064–3076 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Channels, W., Nedelec, F., Zheng, Y. & Iglesias, P. Spatial regulation improves antiparallel microtubule overlap during mitotic spindle assembly. Biophys. J. 94, 2598–2609 (2007).

PubMed  PubMed Central  Google Scholar 

Cheerambathur, D. K. et al. Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient. J. Cell Biol. 177, 995–1004 (2007).

CAS  PubMed  PubMed Central  Google Scholar 

Chalamalasetty, R. B., Hummer, S., Nigg, E. A. & Sillje, H. H. Influence of human Ect2 depletion and overexpression on cleavage furrow formation and abscission. J. Cell Sci. 119, 3008–3019 (2006).

CAS  PubMed  Google Scholar 

Hu, C. K., Coughlin, M., Field, C. M. & Mitchison, T. J. Cell polarization during monopolar cytokinesis. J. Cell Biol. 181, 195–202 (2008).

CAS  PubMed  PubMed Central  Google Scholar 

Höög, J. L. et al. Organization of interphase microtubules in fission yeast analyzed by electron tomography. Dev. Cell 12, 349–361 (2007).

PubMed  Google Scholar 

Drummond, D. R. & Cross, R. A. Dynamics of interphase microtubules in Schizosaccharomyces pombe. Curr. Biol. 10, 766–775 (2000).

CAS  PubMed  Google Scholar 

Tran, P. T., Marsh, L., Doye, V., Inoue, S. & Chang, F. A mechanism for nuclear positioning in fission yeast based on microtubule pushing. J. Cell Biol. 153, 397–411 (2001).

CAS  PubMed  PubMed Central  Google Scholar 

Carazo-Salas, R. E., Antony, C. & Nurse, P. The kinesin Klp2 mediates polarization of interphase microtubules in fission yeast. Science 309, 297–300 (2005).

CAS  PubMed  Google Scholar 

Carazo-Salas, R. E. & Nurse, P. Self-organization of interphase microtubule arrays in fission yeast. Nature Cell Biol. 8, 1102–1107 (2006).

CAS  PubMed  Google Scholar 

Daga, R. R., Lee, K. G., Bratman, S., Salas-Pino, S. & Chang, F. Self-organization of microtubule bundles in anucleate fission yeast cells. Nature Cell Biol. 8, 1108–1113 (2006).

CAS  PubMed  Google Scholar 

Powers, J. et al. Loss of KLP-19 polar ejection force causes misorientation and missegregation of holocentric chromosomes. J. Cell Biol. 166, 991–1001 (2004).

CAS  PubMed  PubMed Central  Google Scholar 

Williams, B. C., Riedy, M. F., Williams, E. V., Gatti, M. & Goldberg, M. L. The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis. J. Cell Biol. 129, 709–723 (1995).

CAS  PubMed  Google Scholar 

Toure, A. et al. MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells. J. Biol. Chem. 273, 6019–6023 (1998).

CAS  PubMed  Google Scholar 

Goldstein, A. Y., Jan, Y. N. & Luo, L. Function and regulation of Tumbleweed (RacGAP50C) in neuroblast proliferation and neuronal morphogenesis. Proc. Natl Acad. Sci. USA 102, 3834–3839 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Nislow, C., Lombillo, V. A., Kuriyama, R. & McIntosh, J. R. A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles. Nature 359, 543–547 (1992).

CAS  PubMed  Google Scholar 

Terada, Y. et al. AIM-1: a mammalian midbody-associated protein required for cytokinesis. EMBO J. 17, 667–676 (1998).

CAS  PubMed  PubMed Central  Google Scholar 

Schumacher, J. M., Golden, A. & Donovan, P. J. AIR-2: An Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos. J. Cell Biol. 143, 1635–1646 (1998).

CAS  PubMed  PubMed Central  Google Scholar 

Cooke, C. A., Heck, M. M. & Earnshaw, W. C. The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis. J. Cell Biol. 105, 2053–2067 (1987).

CAS  PubMed  Google Scholar 

Adams, R. R., Maiato, H., Earnshaw, W. C. & Carmena, M. Essential roles of Drosophila inner centromere protein (INCENP) and Aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation. J. Cell Biol. 153, 865–880 (2001).

CAS  PubMed  PubMed Central  Google Scholar 

Li, F. et al. Pleiotropic cell-division defects and apoptosis induced by interference with survivin function. Nature Cell Biol. 1, 461–466 (1999).

CAS  PubMed  Google Scholar 

Fraser, A. G., James, C., Evan, G. I. & Hengartner, M. O. Caenorhabditis elegans inhibitor of apoptosis protein (IAP) homologue BIR-1 plays a conserved role in cytokinesis. Curr. Biol. 9, 292–301 (1999).

CAS  PubMed  Google Scholar 

Jones, G., Jones, D., Zhou, L., Steller, H. & Chu, Y. Deterin, a new inhibitor of apoptosis from Drosophila melanogaster. J. Biol. Chem. 275, 22157–22165 (2000).

CAS  Google Scholar 

Gassmann, R. et al. Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle. J. Cell Biol. 166, 179–191 (2004).

CAS  PubMed  PubMed Central  Google Scholar 

Sampath, S. C. et al. The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Cell 118, 187–202 (2004).

CAS  PubMed  Google Scholar 

Romano, A. et al. CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1. J. Cell Biol. 161, 229–236 (2003).

CAS  PubMed  PubMed Central  Google Scholar 

Hanson, K. K., Kelley, A. C. & Bienz, M. Loss of Drosophila borealin causes polyploidy, delayed apoptosis and abnormal tissue development. Development 132, 4777–4787 (2005).

CAS  PubMed  Google Scholar 

Lee, K. S., Yuan, Y. L., Kuriyama, R. & Erikson, R. L. Plk is an M-phase-specific protein kinase and interacts with a kinesin- like protein, CHO1/MKLP-1. Mol. Cell Biol. 15, 7143–7151 (1995).

CAS  PubMed  PubMed Central  Google Scholar 

Mundt, K. E., Golsteyn, R. M., Lane, H. A. & Nigg, E. A. On the regulation and function of human polo-like kinase 1 (PLK1): effects of overexpression on cell cycle progression. Biochem. Biophys. Res. Commun. 239, 377–385 (1997).

CAS  PubMed  Google Scholar 

Wilson, G. M. et al. The FIP3–Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis. Mol. Biol. Cell 16, 849–860 (2005).

CAS  PubMed  PubMed Central  Google Scholar 

Tatsumoto, T., Xie, X., Blumenthal, R., Okamoto, I. & Miki, T. Human ECT2 is an exchange factor for rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. J. Cell Biol. 147, 921–928 (1999).

CAS  PubMed  PubMed Central  Google Scholar